СGH - Comparative Genomic Hybridization or CGH is a cytogenetic technique developed for detecting of chromosome aberrations: deletions and amplifications. The tremendous strength of the CGH technique is the capability to analyze all the genomic aberrations within one experiment. is based on comparison of the test DNA and the reference DNA. They are labeled with different fluorochromes, mixed 1:1 and hybridized on metaphase chromosomes from a normal human. Chromosome aberrations are estimated using the difference of fluorescence intensity of two fluorochromes. The so called Fluorescence Ratio (FR) is calculated. The changes in the FR values along the chromosome deliver information on the number of DNA copies in the chromosome region of interest in comparison with the reference DNA.

                                                                                                                                                                            Ana Sayfa  >>>

Automatic Digital
CGH Analyzer

Automatic Digital CGH Analyzer

 

 

 

 

 

 

 

                                                                                                                                             

 

                                                                                                  

CGH technique is widely used for diagnostic purposes.
It is applied in clinical genetics to detect amplifications and deletions
in tumor cells and cells of patients with congenital or acquired chromosomal abnormalities.

 

 

                                                                                                VdeoTesT-CGH system is able to acquire a stack of images of metaphase plate with hybridized DNA (DAPI, FITC, TRITC filters), to calculate fluorescence ratio denoting green fluorescence intensity (test DNA) to red fluorescence intensity (reference DNA) ratio, and to create false-colored mage according to the calculated coefficient.



 

           

                                                                                                                               

 

                                                                                                  Then the corresponding karyogram is automatically constructed, in which standard idiograms of chromosomes can be displayed. The chromosome regions, on which DNA deletions (losses) or amplifications (gains) have been detected, are vizualized directly on chromosomes. For every chromosome, its fluoresecence ratio profile (FITC/TRITC) can be shown. Images and analysis results are saved in in-built database and can be printed out as a customizable form.