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TesT-FISH software is designed to automate the FISH analysis procedure.

 
Fluorescence in Situ Hybridization or FISH is a method of location or DNA existence identification directly on a cytological specimen, i.e. directly in the sites of their real location in a cell or in a chromosome (in situ - directly in the place). Hybridization in situ - is an adaptation of nuclear hybridization method carried out with solutions of nuclear acids extracts. This method is widely used for physical mapping of plant, animal and human chromosomes, for linking of genes and anonymous DNA samples to specific chromosome regions, for detection of different chromosomal rearrangements by using specific molecular DNA probes, for visualization of location of various chromosomal segments in interphasic nuclei, determination of kinship between species.

The TesT-FISH 2.0 is the basis of the specialized TesT-FISH image analysis system.
 
VideoTesT-FISH Software for Automation of the FISH Analysis
VideoTesT-FISH Software:color coding
VideoTesT-FISH Software: automatic karyotyping
VideoTesT-FISH Software: built-in image database
 
VideoTesT-FISH 2.0 main functions:
  • Acquiring stacks of b/w images from TV/digital camera or scanner having a TWAIN driver (8, 10, 12, 16 bit). All the TesT direct camera drivers at your disposal.
  • Correction of optical image shift caused by filter exchange.
  • Color coding of source grayscale images with ability to select colors from palette. Up to 20 color channels can be used.
  • Automated generation of the composed color image. Selecting channels to be combined by clicking their thumbnails in the navigation bar.
  • Multiple display mode: inverted counterstain, single color-coded images,
  • Signal enhancement for individual channels (Brightness/Contrast, Unsharp Mask, Smooth).
  • Background correction, background removal for highlighting the weak signals.
  • Distance measurements in real units (straight line length, freehand line length). Easy process of calibration (stage micrometer is required).
  • Special features for working with chromosomes! Interactive detection of chromosomes on metaphase plate, automatic separation of overlapping chromosomes.
  • Automatic karyotyping of human chromosomes on DAPI image with ability to train classifier. The classifier recognizes chromosomes based on banding pattern.
  • Convenient manual karyotyping of animal and plant chromosomes.
  • Built-in reference databases of human, animal and plant ideograms.
  • Nondestructive annotation on images and karyograms.
  • Interactive report generator for creating multipage reports containing images, karyograms and results of analysis.
  • Export to common image format (jpeg) for exchange with other applications or remote consulting.
  • User-defined number of undoable operations (undo levels) enables flexible correction and security of every step.
  • Saving source images, karyograms and analysis results to built-in image database.
  • Ability to save image stack together with all the analysis results to one file.